Recombinant Human TPO Instruction

General description

Thrombopoietin is the primary cytokine regulating megakaryocyte development and platelet production. It also supports hematopoietic stem cell maintenance and expansion. Recombinant Human TPO Protein (Y07201) is expressed from mammalian cells. It contains Ser 22-Leu 195 (Accession # P40225), and is widely used in advanced cell culture, hematology research, and cell therapy manufacturing.

Application

· Megakaryocyte Differentiation — Drives CD34+ hematopoietic stem and progenitor cells toward megakaryocyte and platelet lineages.

· Hematopoietic Stem Cell Expansion — Supports ex vivo expansion of cord blood and bone marrow HSCs.

· Platelet Production Research — Models in vitro platelet biogenesis for transfusion medicine.

· Gene and Cell Therapy Manufacturing — Used in clinical-grade expansion protocols for HSC-based therapies.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

TPO, also known as THPO or MGDF, is produced primarily in the liver and binds to the MPL receptor on hematopoietic stem cells and megakaryocyte progenitors, activating JAK2-STAT5 signaling. TPO exhibits pleiotropic effects on the hematopoietic system, serving as a critical factor for HSC quiescence and self-renewal. Clinically, recombinant TPO and TPO receptor agonists treat thrombocytopenia. In research and manufacturing, TPO is indispensable for serum-free media formulations for expanding CD34+ cells, generating megakaryocytes, and producing platelets in bioreactor systems.

Q&A

Q1: What is TPO mainly used for?

TPO is primarily used for megakaryocyte differentiation, HSC expansion, platelet production research, and clinical cell therapy manufacturing.

Q2: Is this TPO suitable for serum-free culture systems?

Yes. EastMabBio recombinant TPO is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant TPO be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.