Recombinant Porcine IL-6 Instruction

General description

Interleukin-6 is a pleiotropic cytokine with both pro-inflammatory and anti-inflammatory properties, regulating acute phase responses, B cell differentiation, and Th17 cell development. It is a critical mediator of cytokine release syndrome and a therapeutic target in autoimmune diseases and cancer. Recombinant Porcine IL-6 Protein (Y02321P) is expressed from E. coli cells. It contains AA Glu30-Met212 (Accession # P26893), and is widely used in cell culture, immunology research, and inflammation modeling.

Application

Cell-Cultured Pork Production — Supports the growth and metabolic activity of porcine muscle satellite cells and fibroblasts.

Porcine Immune Response Modeling — Models acute phase reactions and inflammation in porcine disease research.

Porcine B Cell and Plasma Cell Differentiation — Drives antibody production in porcine lymphocyte cultures.

Porcine Mastitis and Infection Research — Investigates IL-6-driven pathology in mammary gland infection.

Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

Q&A

Q1:  What is IL-6 mainly used for?

TNF-α is primarily used for macrophage activation, inflammation signaling research, apoptosis studies, and intestinal organoid modeling.

Q2: Is this IL-6 suitable for serum-free culture systems?

Yes. EastMabBio recombinant IL-6 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant IL-6 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.