Recombinant Human SCF Instruction

General description

Stem Cell Factor is a hematopoietic growth factor essential for the survival, proliferation, and differentiation of hematopoietic stem cells, mast cells, and melanocytes. It signals through the c-Kit receptor tyrosine kinase. Recombinant Human SCF Protein (Y03801) is expressed from CHO cells. It contains AA Glu 26 - Ala 190 (Accession # P21583), and is widely used in advanced cell culture, hematology research, and mast cell biology.

Application

· Hematopoietic Stem Cell Expansion — Supports the ex vivo growth and maintenance of CD34+ cells.

· Mast Cell Differentiation and Culture — Drives the development and maturation of connective tissue mast cells.

· Melanocyte Survival and Proliferation — Promotes the growth of pigment-producing cells.

· Gametogenesis Research — Supports spermatogonial and oocyte development in reproductive biology.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

SCF, also known as c-Kit ligand or steel factor, exists as both soluble and membrane-bound forms generated by alternative splicing. It signals through the c-Kit receptor, activating PI3K-AKT, RAS-MAPK, and PLCγ pathways. SCF is indispensable for hematopoiesis, with genetic mutations causing severe anemia, mast cell deficiency, and sterility. In cell culture, SCF is commonly combined with other early-acting cytokines to maximize HSC expansion and is essential for establishing mast cell cultures from bone marrow or cord blood progenitors.

Q&A

Q1: What is SCF mainly used for?

SCF is primarily used for HSC expansion, mast cell differentiation, melanocyte culture, and gametogenesis research.

Q2: Is this SCF suitable for serum-free culture systems?

Yes. EastMabBio recombinant SCF is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant SCF be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.