Recombinant Human Wnt-3a (hFc Tag） Instruction

General description

Wnt-3a is a canonical Wnt ligand that activates β-catenin-dependent signaling to drive stem cell self-renewal, proliferation, and lineage specification. The human Fc fusion format enhances protein stability and simplifies purification, enabling consistent activity in organoid and stem cell culture applications. Recombinant Human Wnt-3a-hFc Protein (Y04603) is expressed from mammalian cells. It contains AA Ser 19 - Lys 352 with a C-terminal linker GGGGGS and

Human lgG1 (Asp104-Lys330), and is widely used in advanced cell culture, organoid research, and developmental biology.

Application

· Organoid Initiation and Maintenance — Drives canonical Wnt signaling in intestinal, hepatic, and retinal organoid cultures.

· Stem Cell Self-Renewal — Maintains pluripotency and expands tissue-specific stem cell populations.

· Neural Crest Induction — Promotes neural crest specification in pluripotent stem cell differentiation.

· Tumor Organoid Culture — Supports Wnt-dependent cancer stem cell maintenance.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

Wnt-3a, also known as WNT3A, is a member of the Wnt family of secreted glycoproteins that regulate cell fate decisions during embryonic development and adult tissue homeostasis. Canonical Wnt signaling stabilizes β-catenin, enabling its nuclear translocation and TCF/LEF-mediated transcription of target genes including MYC, CCND1, and LGR5. Native Wnt-3a is notoriously difficult to produce recombinantly due to lipid modification requirements and poor solubility. The hFc fusion format addresses these challenges by enhancing protein stability, improving purification efficiency, and extending half-life in culture medium. The Fc domain does not interfere with Wnt receptor binding but provides a convenient handle for protein detection and immobilization. EastMabBio's Wnt-3a-hFc demonstrates robust TOPFlash reporter activity and supports efficient organoid establishment from primary tissue, offering a practical alternative to native Wnt-3a for routine laboratory applications.

Q&A

Q1: What is Wnt-3a-hFc mainly used for?

Wnt-3a-hFc is primarily used for organoid initiation and maintenance, stem cell self-renewal, neural crest induction, and tumor organoid culture.

Q2: Is this Wnt-3a-hFc suitable for serum-free culture systems?

Yes. EastMabBio recombinant Wnt-3a-hFc is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant Wnt-3a-hFc be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.