Recombinant Human EPO Instruction

General description

Erythropoietin is the primary cytokine regulating red blood cell production from erythroid progenitors. It is essential for maintaining oxygen homeostasis and is widely used to treat anemia. Recombinant Human EPO Protein (Y05801) is expressed from mammalian cells. It contains AA Ala 28 - Arg 193 (Accession # P01588), and is widely used in advanced cell culture, hematology research, and erythroid differentiation studies.

Application

· Erythroid Progenitor Differentiation — Drives the maturation of BFU-E and CFU-E into enucleated red blood cells.

· Hematopoietic Stem Cell Culture — Supports erythroid lineage commitment in cord blood and bone marrow cultures.

· Anemia and Erythropoiesis Research — Models erythroid development and therapeutic erythropoietin responses.

· Cell-Cultured Blood Production — Enables the generation of universal donor red blood cells from stem cells.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

EPO is a glycoprotein hormone produced primarily by peritubular fibroblasts in the kidney in response to hypoxia. It signals through the EPOR homodimer, activating JAK2-dependent STAT5, PI3K-AKT, and RAS-MAPK pathways to promote erythroid progenitor survival, proliferation, and terminal differentiation. Proper glycosylation is essential for EPO in vivo half-life and bioactivity, making mammalian expression critical for research and therapeutic applications. In cell culture, EPO is indispensable for erythroid differentiation protocols and the emerging field of cultured red blood cell production.

Q&A

Q1: What is EPO mainly used for?

EPO is primarily used for erythroid progenitor differentiation, HSC culture, anemia research, and cell-cultured blood production.

Q2: Is this EPO suitable for serum-free culture systems?

Yes. EastMabBio recombinant EPO is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant EPO be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.