Recombinant Human FLT-3L Instruction

General description

FMS-Like Tyrosine Kinase 3 Ligand is a hematopoietic growth factor that stimulates the proliferation and differentiation of early hematopoietic progenitors and dendritic cells. It is essential for immune cell development and expansion. Recombinant Human FLT-3L Protein (Y01201) is expressed from mammalian cells. It contains AA Thr 27 - Pro 184 (Accession # P49771), and is widely used in advanced cell culture, immunology research, and dendritic cell generation.

Application

· Hematopoietic Stem Cell Expansion — Supports the ex vivo expansion of CD34+ stem and progenitor cells.

· Dendritic Cell Differentiation — Drives the generation of conventional and plasmacytoid dendritic cells from progenitors.

· Immunotherapy Research — Enhances antigen-presenting cell function for vaccine and cancer immunotherapy.

· Lymphoid Progenitor Development — Promotes B cell and NK cell precursor proliferation.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

FLT-3L is a type I transmembrane protein that can be cleaved to generate a soluble form. It signals through the FLT-3 receptor tyrosine kinase, activating PI3K-AKT, RAS-MAPK, and STAT5 pathways to promote hematopoietic progenitor survival and proliferation. FLT-3L is indispensable for early hematopoiesis, with genetic deletion causing profound deficiencies in dendritic cells, B cells, and NK cells. In cell culture, FLT-3L is commonly used in combination with other cytokines to expand CD34+ cells and to generate large numbers of dendritic cells for immunotherapy applications.

Q&A

Q1: What is FLT-3L mainly used for?

FLT-3L is primarily used for HSC expansion, dendritic cell differentiation, immunotherapy research, and lymphoid progenitor development.

Q2: Is this FLT-3L suitable for serum-free culture systems?

Yes. EastMabBio recombinant FLT-3L is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant FLT-3L be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.