Recombinant Human IL-4 Instruction

General description

Interleukin-4 is a Th2-polarizing cytokine that regulates humoral immunity, allergic responses, and alternative macrophage activation. It is essential for B cell class switching, eosinophil recruitment, and the suppression of Th1 inflammatory pathways. Recombinant Human IL-4 Protein (Y02201) is expressed from mammalian cells. It contains AA His 25 - Ser 153 (Accession # P05112), and is widely used in advanced cell culture, immunology research, and allergy modeling.

Application

•  Th2 Cell Differentiation — Drives polarization of naïve CD4+ T cells toward IL-4-secreting Th2 effector populations.

• B Cell Activation and Class Switching — Promotes IgE and IgG1 production in activated B lymphocytes.

• M2 Macrophage Polarization — Induces alternative activation of macrophages toward tissue repair phenotypes.

• Dendritic Cell Maturation — Supports the generation of mature antigen-presenting cells for vaccine research.

• Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

• 36 months at -20°C to -80°C in lyophilized state;

• 6 months at -20°C to -80°C under sterile conditions after reconstitution;

• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

• Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

• It is recommended to redissolve in sterile deionized water.

Backgroud

Interleukin-4, also known as IL-4 or B cell stimulatory factor-1, signals through the type I receptor complex composed of IL-4Rα and the common γ-chain, or the type II complex containing IL-4Rα and IL-13Rα1. This dual receptor usage explains the overlapping biological activities of IL-4 and IL-13 while preserving distinct signaling capacities in different cell types. IL-4 activates JAK1/JAK3-dependent STAT6 signaling that drives the master transcription factor GATA3, committing naïve T cells to the Th2 lineage and suppressing Th1 differentiation through downregulation of T-bet and STAT1 activity. In B cells, IL-4 promotes class switch recombination to IgE and IgG1 isotypes, underpinning allergic sensitization and humoral defense against helminth parasites. In macrophage biology, IL-4 induces alternative activation toward the M2 phenotype characterized by arginase-1 expression, mannose receptor upregulation, and tissue repair functions. EastMabBio's IL-4 is validated for Th2 polarization efficiency in primary human T cell cultures and supports reproducible dendritic cell maturation protocols for cancer vaccine development.

Q&A

Q1: What is IL-4 mainly used for?

IL-4 is primarily used for Th2 cell differentiation, B cell class switching, M2 macrophage polarization, and dendritic cell maturation.

Q2: Is this IL-4 suitable for serum-free culture systems?

Yes. EastMabBio recombinant IL-4 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant IL-4 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.