Recombinant Bovine LIF Instruction

General description

Leukemia Inhibitory Factor is a pleiotropic cytokine that maintains pluripotency in mouse embryonic stem cells and regulates hematopoiesis, neuronal development, and inflammation. It signals through the gp130 receptor complex. Recombinant Bovine LIF Protein (Y03021B) is expressed from E.coli cells. It contains AA Ser 23 - Phe 202 (Accession # Q27956), and is widely used in cell agriculture, developmental biology, and veterinary biotechnology.

Application

· Bovine Embryonic Stem Cell Pluripotency Maintenance — Prevents differentiation and sustains self-renewal in bESC culture.

· Cell-Cultured Beef Production — Supports the expansion and genetic stability of bovine pluripotent stem cells.

· Bovine Cloning and Nuclear Transfer — Enhances the efficiency of somatic cell nuclear transfer protocols.

· Bovine Embryo Development Research — Models early embryogenesis and inner cell mass formation.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

LIF is a member of the IL-6 cytokine family that signals through the LIFRβ/gp130 receptor complex, activating JAK-STAT3 and MAPK pathways. It was originally identified by its ability to induce macrophage differentiation in leukemia cells and subsequently recognized as a critical factor for maintaining mouse embryonic stem cell pluripotency. LIF is not required for human ESC pluripotency but supports hematopoietic progenitor expansion, neuronal differentiation, and trophoblast implantation. In cell culture, LIF is indispensable for standard mESC maintenance protocols.

Q&A

Q1: What is  LIF mainly used for?

LIF is primarily used for HSC expansion, dendritic cell differentiation, immunotherapy research, and lymphoid progenitor development.

Q2: Is this LIF suitable for serum-free culture systems?

Yes. EastMabBio recombinant LIF is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant LIF be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.