Recombinant Human β-NGF(HEK293-expressed)

General Description

Beta-Nerve Growth Factor is the prototypical neurotrophin that supports the survival, differentiation, and axonal outgrowth of sympathetic and sensory neurons. It is essential for the development and maintenance of the peripheral nervous system. Recombinant Human β-NGF Protein (Y06941H) is expressed from mammalian cells. It contains AA Ser 122 - Arg 241 (Accession # P01138), and is widely used in advanced cell culture, neuroscience research, and peripheral nerve studies.

Application

· Sympathetic and Sensory Neuron Culture — Supports the survival and neurite outgrowth of dorsal root ganglion neurons.

· Cholinergic Basal Forebrain Neuron Maintenance — Promotes survival of septal and hippocampal cholinergic neurons.

· Inflammation and Pain Research — Models NGF-mediated nociceptor sensitization and chronic pain mechanisms.

· Neural Crest Cell Differentiation — Drives the development of autonomic and sensory neuronal lineages.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

β-NGF is a homodimeric neurotrophin that signals through TrkA and p75NTR receptors to activate PI3K-AKT, PLCγ, and MAPK pathways. It was the first discovered neurotrophin, identified by its ability to promote dorsal root ganglion neuron survival. NGF is produced by target tissues of sympathetic and sensory innervation and functions as a retrograde survival factor. Beyond neurotrophic effects, NGF plays roles in immune regulation, inflammation, and pain sensitization, with therapeutic antibodies targeting NGF (tanezumab) developed for chronic pain conditions. In cell culture, β-NGF is essential for maintaining primary sympathetic and sensory neurons and for differentiating neural crest cells toward autonomic lineages.

Q&A

Q1: What is β-NGF mainly used for?

β-NGF is primarily used for sympathetic and sensory neuron culture, cholinergic neuron maintenance, pain research, and neural crest differentiation.

Q2: Is this β-NGF suitable for serum-free culture systems?

Yes. EastMabBio recombinant β-NGF is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant β-NGF be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.