Recombinant Human FGF-9

General description

Fibroblast Growth Factor 9 is a mesenchymally expressed growth factor that regulates chondrogenesis, lung development, and male germ cell survival. It plays essential roles in skeletal patterning, alveolar formation, and spermatogenesis through distinct receptor specificities. Recombinant Human FGF-9 Protein (Y01001) is expressed from mammalian cells. It contains AA Pro 3 - Ser 208 (Accession # P31371), and is widely used in advanced cell culture, developmental biology, and reproductive biology research.

Application

· Keratinocyte and Epithelial Cell Proliferation — Drives growth of skin, oral, and corneal epithelial cells.

· Wound Healing and Re-epithelialization — Accelerates closure of cutaneous and mucosal wounds.

· Radiation-Induced Mucositis Protection — Models tissue-sparing effects in oral and intestinal mucosa.

· Lung Epithelial Repair — Supports alveolar type II cell proliferation and surfactant production.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

Fibroblast Growth Factor 9, also known as FGF-9 or GAF, is a member of the FGF family that signals primarily through FGFR2 and FGFR3 receptor isoforms. Unlike most FGFs, FGF-9 exhibits high-affinity binding to both FGFR2IIIb and FGFR2IIIc isoforms, as well as FGFR3IIIc, creating unique signaling capabilities that distinguish it from other family members. During embryonic development, FGF-9 is expressed in the mesenchyme of developing limbs, lungs, and testes, where it regulates chondrogenesis, branching morphogenesis, and germ cell survival through paracrine signaling to adjacent epithelial compartments. In the lung, FGF-9 is essential for mesenchymal proliferation and alveolar septation, with genetic deletion causing severe pulmonary hypoplasia. In the testis, FGF-9 maintains spermatogonial stem cells and promotes Sertoli cell proliferation, making it critical for male fertility. In skeletal biology, FGF-9 stimulates chondrocyte proliferation and matrix synthesis while inhibiting hypertrophic differentiation, suggesting therapeutic potential for cartilage repair in osteoarthritis. EastMabBio's FGF-9 is validated for chondrocyte proliferation assays, lung epithelial-mesenchymal co-culture systems, and spermatogonial stem cell maintenance, supporting diverse research applications in developmental biology and regenerative medicine.

Q&A

Q1:  What is FGF-9 mainly used for?

FGF-9 is primarily used for chondrocyte culture, lung development research, male germ cell culture, and osteoarthritis studies.

Q2: Is this FGF-9 suitable for serum-free culture systems?

Yes. EastMabBio recombinant FGF-9 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant FGF-9 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.