Recombinant Human FGF-1/aFGF

General description

Acidic Fibroblast Growth Factor is a potent mitogen and survival factor for fibroblasts, endothelial cells, and neural progenitors. It exhibits broad receptor specificity and strong affinity for heparin, making it a versatile tool for cell culture and tissue engineering applications. Recombinant Human FGF-1 Protein (Y05421NH) is expressed from E. coli cells. It contains AA Phe 16 - Asp 155 (Accession # NP_001127073.1), and is widely used in advanced cell culture, angiogenesis research, and neural development studies.

Application

· Fibroblast and Endothelial Cell Proliferation — Drives growth of mesenchymal and vascular cell types in serum-free conditions.

· Neural Stem Cell Survival and Differentiation — Supports the maintenance and neuronal commitment of CNS progenitors.

· Angiogenesis and Vascular Sprouting — Models blood vessel formation in endothelial tube formation and spheroid assays.

· Wound Healing and Tissue Repair — Promotes granulation tissue formation and re-epithelialization.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

Fibroblast Growth Factor 1, also known as FGF-1, aFGF, or endothelial cell growth factor, is the founding member of the FGF family. Unlike most FGFs, FGF-1 lacks a classical signal peptide and is released through non-classical secretion pathways or from damaged cells. It binds with high affinity to all four FGF receptors (FGFR1, FGFR2, FGFR3, and FGFR4) in the presence of heparan sulfate proteoglycans, activating RAS-MAPK, PI3K-AKT, and PLCγ signaling cascades. This broad receptor specificity underlies the wide-ranging biological activities of FGF-1, which include stimulation of fibroblast proliferation, endothelial cell migration and tube formation, neural cell survival, and cardiomyocyte protection. FGF-1 is particularly valuable in cell culture applications requiring strong mitogenic stimulation without the lineage-restricted effects of more specialized growth factors. Its acidic isoelectric point distinguishes it from basic FGF-2 and influences its heparin-binding properties and tissue distribution. EastMabBio's FGF-1 is validated for endothelial cell proliferation, neural stem cell maintenance, and fibroblast growth assays, providing researchers with a reliable source of this foundational growth factor.

Q&A

Q1:  What is FGF-1/aFGF mainly used for?

FGF-1/aFGF is primarily used for fibroblast and endothelial cell proliferation, neural stem cell culture, angiogenesis research, and wound healing studies.

Q2: Is this FGF-1/aFGF suitable for serum-free culture systems?

Yes. EastMabBio recombinant FGF-1/aFGF is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant FGF-1/aFGF be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.