Recombinant Human NRG1-β1

General Description

Neuregulin 1 Beta 1 is a member of the epidermal growth factor family that regulates neuronal development, myelination, and cardiac muscle differentiation. It signals through ErbB receptor tyrosine kinases to promote survival and maturation of diverse cell types. Recombinant Human NRG1-β1 Protein (Y01621H) is expressed from E. coli cells. It contains S 177-E 241(Accession # NP_039250), and is widely used in advanced cell culture, neuroscience research, and cardiac biology.

Application

· Schwann Cell and Oligodendrocyte Differentiation — Promotes myelination and glial cell maturation.

· Neuronal Survival and Synapse Formation — Supports the development and maintenance of peripheral and central neurons.

· Cardiomyocyte Differentiation and Survival — Enhances the maturation and electrical coupling of stem cell-derived cardiac cells.

· Breast Cancer and ErbB Signaling Research — Models NRG1-driven tumor progression and therapeutic resistance.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

NRG1-β1, also known as heregulin-β1 or glial growth factor, is a soluble isoform of Neuregulin 1 that contains an EGF-like domain essential for ErbB receptor binding. It signals through ErbB3/ErbB2 and ErbB4 receptor complexes, activating PI3K-AKT and RAS-MAPK pathways to promote cell survival, proliferation, and differentiation. NRG1-β1 is critical for peripheral nervous system myelination, cardiac development, and the maintenance of neuromuscular junctions. In cell culture, it is indispensable for Schwann cell expansion, oligodendrocyte maturation, and the generation of electrically active cardiomyocytes from pluripotent stem cells.

Q&A

Q1: What is NRG1-β1 mainly used for?

NRG1-β1 is primarily used for cell-cultured pork production, wound healing research, intestinal organoid culture, and mammary gland development.

Q2: Is this NRG1-β1 suitable for serum-free culture systems?

Yes. EastMabBio recombinant NRG1-β1 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant NRG1-β1 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.