Y04603 Recombinant Human Wnt-3a (hFc Tag) Product Brochure

Cat#:Y04603

Name:Y04603 Recombinant Human Wnt-3a (hFc Tag)

Species:Human

Specification:10μg/50μg/1mg

Purity:≥95%

Endotoxin: ≤100 EU/mg

Expression System:CHO

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Product Details
Data
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Product Details

Cat. No.

Y04603

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).

Endotoxin

≤100 EU/mg by the LAL method.

Bioactivity

Measured by its ability to induce Topflash reporterBioactivity in CHO-K1 cells. The ED₅₀ for this effect is ＜30 ng/mL.

Accession

NP_149122

Alternative Names

MG/VAIL C119418; MG/VAIL C119419; MG/VAIL C119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a

Species

Human

Expression System

CHO

Mol. Weight

58.2 kDa

Expression Region

Ser19-Lys352 with a C-terminal linker GGGGGS and Human lgG1 (Asp104-Lys330)

Tag Info

hFc Tag

Formulation

Lyophilized

Buffer

Lyophilized from a 0.22 μm filtered solution containing PBS, pH7.4.

Size

10μg/50μg/1mg

Lead Time

Products are in stock, but custom products may require further consultation.

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Data

SDS-PAGE

Y04603 Recombinant Human Wnt-3a(hFc Tag).jpg

Recombinant Human Wnt-3a(hFc Tag) (Cat. No. Y04603) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04603 SDS-PAGE.

Bioactivity

Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 5 ng/mL BMP-2. The ED₅₀ for this effect is ＜30 ng/mL. The bioactivity of Recombinant Human Wnt-3a (hFc Tag) (Cat. No. Y04603) was higher than other competing products.

Bioactivity

Highly consistent bioactivity between different production batches of Recombinant Human Wnt-3a (hFc Tag) (Cat. No. Y04063).