Recombinant Human Laminin521 E8 Instruction

General description

Laminin 521 is a trimeric basement membrane protein that provides an optimal microenvironment for the clonal expansion and genetic stability of human pluripotent stem cells. It enables efficient single-cell culture without ROCK inhibitor supplementation. Recombinant Human Laminin 521 Protein (Y13342HL) is expressed from mammalian cells. It contains the α5, β2, and γ1 chains (Accession # O15230, P55268, P11047), and is widely used in advanced cell culture, stem cell research, and cell therapy manufacturing.

Application

· Human Pluripotent Stem Cell Expansion — Supports clonal growth and single-cell passaging without ROCK inhibitor.

· Gene Editing and Clonal Selection — Enables efficient single-cell cloning for genetic modification.

· Cell Therapy Manufacturing — Provides a defined, GMP-compatible substrate for clinical-grade hPSC culture.

· Neural and Retinal Progenitor Culture — Supports the expansion of lineage-restricted progenitor populations.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

Laminin 521 is expressed in the human embryo and provides the most supportive niche for hPSC survival and self-renewal. It binds to integrin α6β1 with higher affinity than laminin 511, activating robust FAK and PI3K-AKT signaling that suppresses anoikis and enables single-cell survival without ROCK inhibitor. This property makes laminin 521 indispensable for gene editing, clonal selection, and cell therapy manufacturing workflows requiring single-cell manipulation.

Q&A

Q1: What is Laminin 521 mainly used for?

Laminin 521 is primarily used for hPSC expansion, single-cell cloning, cell therapy manufacturing, and neural progenitor culture.

Q2: Is this Laminin 521 suitable for serum-free culture systems?

Yes. EastMabBio recombinant Laminin 521 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant Laminin 521 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.