Recombinant Human Laminin511 E8 Instruction

General description

Laminin 511 is a trimeric basement membrane protein that provides an optimal niche for maintaining pluripotency and supporting the expansion of human pluripotent stem cells. It recapitulates the native stem cell microenvironment with high fidelity. Recombinant Human Laminin 511 Protein (Y13242HL) is expressed from mammalian cells. It contains the α5, β1, and γ1 chains (Accession # O15230, P07942, P11047), and is widely used in advanced cell culture, stem cell research, and regenerative medicine.

Application

· Human Pluripotent Stem Cell Culture — Supports feeder-free, xeno-free maintenance of iPSCs and ESCs.

· Neural Stem Cell and Progenitor Expansion — Provides a physiologically relevant niche for CNS-derived cells.

· Hepatocyte and Pancreatic Cell Maintenance — Supports the culture of endoderm-derived differentiated cells.

· Tissue Engineering and Organoid Development — Forms basement membrane scaffolds for 3D culture systems.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

Laminin 511 is a major component of the basement membrane in the inner cell mass and epiblast, making it the physiologically relevant substrate for human pluripotent stem cells. It binds to integrin α6β1 on hPSCs, activating FAK and PI3K-AKT signaling that maintains pluripotency marker expression. Compared to Matrigel or vitronectin, laminin 511 provides a more authentic stem cell niche, enabling single-cell passaging, improved survival, and enhanced genetic stability during long-term culture.

Q&A

Q1: What is Laminin 511 mainly used for?

Laminin 511 is primarily used for human pluripotent stem cell culture, neural stem cell expansion, hepatocyte maintenance, and organoid development.

Q2: Is this Laminin 511 suitable for serum-free culture systems?

Yes. EastMabBio recombinant Laminin 511 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant Laminin 511 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.