Recombinant Human Tissue Factor Instruction

General description

Tissue Factor is the primary initiator of the extrinsic coagulation cascade and plays critical roles in hemostasis, thrombosis, and cell signaling. It is a transmembrane glycoprotein that binds Factor VIIa to activate downstream clotting proteases. Recombinant Human Tissue Factor Protein (Y04301) is expressed from mammalian cells. It contains AA S33-E251 (Accession # P13726), and is widely used in advanced cell culture, coagulation research, and drug development.

Application

· Coagulation Cascade Activation — Initiates the extrinsic pathway in clotting assays and thrombin generation tests.

· Thrombosis and Hemostasis Research — Models TF-driven clot formation and vascular occlusion.

· Cancer Procoagulant Activity — Investigates tumor cell TF expression and metastasis.

· Drug Development and Anticoagulant Screening — Evaluates therapeutic inhibitors of the TF-FVIIa complex.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

Tissue Factor, also known as thromboplastin or CD142, is a 263-amino acid type I transmembrane glycoprotein. It consists of an extracellular domain that binds Factor VII/VIIa, a transmembrane domain, and a short cytoplasmic tail involved in cell signaling. The TF-FVIIa complex activates Factor X and Factor IX, initiating the common coagulation pathway. Beyond hemostasis, TF-FVIIa signaling through protease-activated receptors (PARs) promotes cell migration, angiogenesis, and inflammation. In cancer, TF expression correlates with metastasis and poor prognosis. In research, recombinant TF is used in clotting assays, thrombin generation tests, and drug screening.

Q&A

Q1: What is Tissue Factor mainly used for?

Tissue Factor is primarily used for coagulation cascade activation, thrombosis research, cancer procoagulant studies, and anticoagulant drug screening.

Q2: Is this Tissue Factor suitable for serum-free culture systems?

Yes. EastMabBio recombinant Tissue Factor is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant Tissue Factor be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.