Recombinant Human IL-15 Instruction

General description

Interleukin-15 is a homeostatic cytokine essential for NK cell, NKT cell, and memory CD8+ T cell development and survival. It exhibits potent anti-tumor activity and serves as a critical factor for generating durable adoptive cell therapy products. Recombinant Human IL-15 Protein (Y02701) is expressed from mammalian cells. It contains AA Asn 49 - Ser 162 (Accession # P40933), and is widely used in advanced cell culture, immunotherapy research, and cell therapy manufacturing.

Application

· NK Cell and CD8+ Memory T Cell Maintenance — Supports survival and proliferation of cytotoxic lymphocyte populations.

· CAR-T and TCR-T Cell Therapy Expansion — Enhances stem cell memory phenotype and in vivo persistence.

· Anti-Tumor Immunity Research — Models IL-15-mediated tumor rejection and immune cell activation.

· NKT Cell and Intraepithelial Lymphocyte Culture — Promotes the development and function of unconventional T cell subsets.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

Interleukin-15, also known as IL-15, is a four-helix bundle cytokine that signals through a receptor complex composed of IL-15Rα, IL-2/IL-15Rβ, and the common γ-chain. Unlike IL-2, which is secreted as a soluble factor, IL-15 is primarily presented in trans by IL-15Rα expressed on dendritic cells and monocytes, creating a localized signaling niche that preferentially supports NK cells and memory CD8+ T cells over regulatory T cells. This distinctive presentation mechanism underlies the non-redundant role of IL-15 in maintaining cytotoxic lymphocyte populations and its potential as a cancer immunotherapy with reduced toxicity compared to IL-2. IL-15 activates JAK1/JAK3-dependent STAT5 signaling that promotes cell survival through Bcl-2 upregulation and cell cycle progression through cyclin modulation. In cell therapy manufacturing, IL-15 in combination with IL-7 has emerged as a preferred cytokine cocktail for generating T cell and NK cell products with enhanced durability, stem cell memory characteristics, and therapeutic potency in adoptive transfer settings.

Q&A

Q1:  What is IL-15 mainly used for?

IL-15 is primarily used for NK cell and memory T cell maintenance, CAR-T cell therapy expansion, anti-tumor immunity research, and NKT cell culture.

Q2: Is this IL-15 suitable for serum-free culture systems?

Yes. EastMabBio recombinant IL-15 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant IL-15 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.