Recombinant Human IL-2 Instruction

General Description

Interleukin-2 is the foundational cytokine for T cell proliferation, survival, and effector differentiation. It serves as the primary growth factor for T lymphocytes and is essential for regulatory T cell maintenance and immune tolerance. Recombinant Human IL-2 Protein (Y02001N) is expressed from mammalian cells. It contains AA Ala 21 - Thr 153 (Accession # P60568-1), and is widely used in advanced cell culture, immunotherapy research, and cell therapy manufacturing.

Application

· T Cell and NK Cell Expansion — Drives robust proliferation of activated T lymphocytes and natural killer cells.

· Regulatory T Cell Culture — Supports the expansion and maintenance of FOXP3+ suppressor T cell populations.

· CAR-T and TCR-T Cell Therapy Manufacturing — Enables clinical-scale expansion of therapeutic T cell products.

· Cancer Immunotherapy Research — Models IL-2-based immunotherapy responses and combination strategies.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

Interleukin-2, also known as IL-2 or T cell growth factor, was originally identified by its ability to sustain long-term T cell proliferation in vitro. It signals through the heterotrimeric IL-2 receptor composed of α (CD25), β (CD122), and γ (CD132) chains, activating JAK1/JAK3-dependent STAT5 signaling that promotes cell cycle progression through upregulation of cyclins and survival factors. The high-affinity αβγ complex is expressed on activated T cells, while the intermediate-affinity βγ complex is constitutively present on NK cells and memory T cells. IL-2 occupies a unique position in immunology as both a driver of effector T cell responses and an essential maintenance factor for regulatory T cells that suppress autoimmunity. In cell therapy manufacturing, IL-2 remains the cornerstone of T cell expansion protocols, supporting the clinical-scale production of CAR-T, TCR-T, and tumor-infiltrating lymphocyte products. EastMabBio's IL-2 is manufactured to GMP-compatible standards with validated potency in primary T cell proliferation assays, ensuring consistent performance across research and translational applications.

Q&A

Q1: What is IL-2 mainly used for?

IL-2 is primarily used for T cell and NK cell expansion, regulatory T cell culture, CAR-T cell therapy manufacturing, and cancer immunotherapy research.

Q2: Is this IL-2 suitable for serum-free culture systems?

Yes. EastMabBio recombinant IL-2 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant IL-2 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.