Recombinant Human Transferrin (HOLO) Instruction

General description

Holo-Transferrin is the iron-loaded form of transferrin, an essential iron-transport protein that delivers ferric iron to proliferating cells while maintaining low free iron concentrations. It is a critical component of serum-free and chemically defined cell culture media. Recombinant Human Holo-Transferrin Protein (Y04501) is expressed from mammalian cells. It contains AA Val 20 - Pro 698 (Accession # P02787), and is widely used in advanced cell culture, bioprocessing, and cell therapy manufacturing.

Application

· Serum-Free Media Formulation — Replaces serum-derived transferrin in chemically defined culture systems.

· Iron Delivery and Cell Proliferation — Supports robust growth of iron-dependent cell lines and primary cells.

· Hybridoma and CHO Cell Culture — Enhances monoclonal antibody and recombinant protein production.

· Cell Therapy Manufacturing — Used in clinical-grade, xeno-free media for therapeutic cell expansion.

· Stem Cell and Organoid Culture — Maintains iron homeostasis in pluripotent stem cell and organoid protocols.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

Transferrin, also known as TF or DMT1, is a plasma glycoprotein synthesized primarily in the liver. It binds two ferric iron atoms with high affinity and delivers them to cells via transferrin receptor-mediated endocytosis. The holo form contains bound iron, making it immediately bioavailable to cultured cells without requiring additional iron supplementation. In cell culture, transferrin is indispensable for serum-free formulations because it provides the iron necessary for DNA synthesis, electron transport, and oxygen metabolism while preventing toxic free iron accumulation. Holo-transferrin is particularly valuable for applications requiring precise iron control, including stem cell maintenance, organoid development, and regulatory-compliant cell therapy production where batch-to-batch consistency of iron delivery is paramount.

Q&A

Q1: What is Holo-Transferrin mainly used for?

Holo-Transferrin is primarily used for serum-free media formulation, iron delivery in cell culture, bioprocessing, and clinical cell therapy manufacturing.

Q2: Is this Holo-Transferrin suitable for serum-free culture systems?

Yes. EastMabBio recombinant Holo-Transferrin is specifically designed for serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant Holo-Transferrin be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.