Recombinant Human IL-18（Yeast-expressed, His Tag) Instruction

General description

Interleukin-18 is a pro-inflammatory cytokine structurally related to IL-1 that amplifies IFN-γ production and drives Th1 and NK cell responses. It is a critical mediator of inflammasome activation and serves as a bridge between innate immune recognition and adaptive cell-mediated immunity. Recombinant Human IL-18 Protein (Y02812H) is expressed from Yeast cells. It contains AA Tyr 37 - Asp 193 (Accession # Q14116), and is widely used in advanced cell culture, immunology research, and inflammatory disease modeling.

Application

· Th1 Cell and NK Cell IFN-γ Induction — Synergizes with IL-12 to drive potent interferon-gamma production.

· Inflammasome and Pyroptosis Research — Models caspase-1-dependent IL-18 maturation and release.

· Autoimmune and Inflammatory Disease Studies — Investigates IL-18-driven pathology in rheumatoid arthritis and Crohn's disease.

· Anti-Tumor Immunity Enhancement — Promotes cell-mediated immune responses against solid tumors.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

Interleukin-18, also known as IL-18 or interferon-gamma inducing factor, is synthesized as an inactive 24 kDa pro-protein that requires proteolytic cleavage by caspase-1 within the inflammasome complex to generate the biologically active 18 kDa mature form. This shared activation mechanism with IL-1β positions IL-18 as a key output of inflammasome signaling, though its biological effects are distinct due to unique receptor usage. IL-18 signals through the IL-18Rα/IL-18Rβ heterodimeric receptor, activating MyD88-dependent NF-κB and MAPK pathways similar to IL-1 signaling. The biological potency of IL-18 is regulated by the endogenous antagonist IL-18 binding protein, which is upregulated in response to IFN-γ to create a negative feedback loop. IL-18 synergizes powerfully with IL-12 to drive Th1 differentiation and NK cell IFN-γ production, making it indispensable for cell-mediated immunity against intracellular pathogens and tumors. In research settings, IL-18 is employed to study inflammasome biology, model autoimmune and autoinflammatory diseases, enhance anti-tumor immune responses in combination with IL-12, and investigate the cytokine networks that govern protective versus pathological inflammation.

Q&A

Q1:  What is IL-18 mainly used for?

IL-18 is primarily used for IFN-γ induction, inflammasome research, autoimmune disease modeling, and anti-tumor immunity enhancement.

Q2: Is this IL-18 suitable for serum-free culture systems?

Yes. EastMabBio recombinant IL-18 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant IL-18 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.