Recombinant Human BDNF Instruction

General Description

Brain-Derived Neurotrophic Factor is a member of the neurotrophin family that supports the survival, differentiation, and synaptic plasticity of central and peripheral neurons. It is critical for hippocampal function, learning, and memory. Recombinant Human BDNF Protein (Y00201) is expressed from mammalian cells. It contains AA His 129-Arg 247 (Accession # P23560), and is widely used in advanced cell culture, neuroscience research, and neuroregeneration studies.

Application

· Neuronal Survival and Differentiation — Promotes the development and maintenance of cortical, hippocampal, and sensory neurons.

· Synaptic Plasticity and Long-Term Potentiation — Models learning and memory mechanisms in neuronal cultures.

· Neurodegenerative Disease Research — Investigates BDNF loss in Alzheimer's disease, Parkinson's disease, and depression.

· Neural Organoid Maturation — Supports the development of synaptically active cerebral organoids.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

NRG1-β1, also known as heregulin-β1 or glial growth factor, is a soluble isoform of Neuregulin 1 that contains an EGF-like domain essential for ErbB receptor binding. It signals through ErbB3/ErbB2 and ErbB4 receptor complexes, activating PI3K-AKT and RAS-MAPK pathways to promote cell survival, proliferation, and differentiation. NRG1-β1 is critical for peripheral nervous system myelination, cardiac development, and the maintenance of neuromuscular junctions. In cell culture, it is indispensable for Schwann cell expansion, oligodendrocyte maturation, and the generation of electrically active cardiomyocytes from pluripotent stem cells.

Q&A

Q1: What is BDNF mainly used for?

BDNF is primarily used for neuronal survival and differentiation, synaptic plasticity research, neurodegenerative disease modeling, and neural organoid maturation.

Q2: Is this BDNF suitable for serum-free culture systems?

Yes. EastMabBio recombinant BDNF is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant BDNF be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.