Recombinant Human GDNF Instruction

General Description

Glial Cell Line-Derived Neurotrophic Factor is a potent survival factor for dopaminergic neurons, motor neurons, and enteric neurons. It signals through the RET receptor tyrosine kinase and has demonstrated therapeutic potential for Parkinson's disease and spinal cord injury. Recombinant Human GDNF Protein (Y01421H) is expressed from E. coli cells. It contains AA Ser 78 - Ile 211 (Accession # NP_000505.1), and is widely used in advanced cell culture, neuroscience research, and neuroregeneration studies.

Application

· Dopaminergic Neuron Survival and Differentiation — Supports the development and protection of midbrain dopaminergic neurons.

· Motor Neuron Culture and Protection — Promotes survival of spinal cord motor neurons in ALS and injury models.

· Enteric Nervous System Development — Models gut neural crest-derived neuron development and Hirschsprung disease.

· Kidney and Ureteric Bud Organoid Culture — Supports branching morphogenesis in renal organoid systems.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

GDNF is the founding member of the GDNF family ligands (GFLs) that signal through a unique receptor system composed of the GFRα1 co-receptor and the RET tyrosine kinase. It was originally purified based on its ability to promote dopaminergic neuron survival and has become one of the most extensively studied neurotrophic factors for Parkinson's disease. GDNF also supports motor neurons, enteric neurons, and kidney development, with genetic deletion causing neonatal lethality due to renal agenesis and enteric nervous system deficiency. In cell culture, GDNF is essential for maintaining dopaminergic neurons, expanding enteric neural crest cells, and generating kidney organoids with branching collecting duct systems.

Q&A

Q1: What is GDNF mainly used for?

GDNF is primarily used for dopaminergic neuron survival, motor neuron protection, enteric nervous system development, and kidney organoid culture.

Q2: Is this GDNF suitable for serum-free culture systems?

Yes. EastMabBio recombinant GDNF is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant GDNF be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.