Recombinant Human CNTF Instruction

General Description

Ciliary Neurotrophic Factor is a neurotrophic cytokine that promotes the survival and differentiation of motor neurons, astrocytes, and retinal cells. It signals through the LIF receptor complex and has demonstrated neuroprotective potential in neurodegenerative disease models. Recombinant Human CNTF Protein (Y05621H) is expressed from E. coli cells. It contains AA Met 1 - Met 200 (Accession # NP_000605.1), and is widely used in advanced cell culture, neuroscience research, and neuroprotection studies.

Application

· Motor Neuron Survival and Differentiation — Supports the development and maintenance of spinal cord motor neurons.

· Astrocyte Activation and Culture — Promotes astrocyte proliferation and reactive gliosis modeling.

· Retinal Ganglion Cell Protection — Models neuroprotective effects in optic nerve injury and glaucoma.

· Neurodegenerative Disease Research — Investigates CNTF-mediated protection in ALS and multiple sclerosis models.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

CNTF is a member of the IL-6 cytokine family that signals through a receptor complex composed of CNTFRα, LIFRβ, and gp130, activating JAK-STAT and MAPK pathways. Despite its name, CNTF is not restricted to ciliary neurons but supports diverse neuronal and glial populations. It was originally identified as a survival factor for chick ciliary ganglion neurons and has since been recognized for its potent effects on motor neurons, astrocytes, and retinal cells. CNTF has been explored clinically for amyotrophic lateral sclerosis and weight loss disorders due to its neuroprotective and anorexigenic properties.

Q&A

Q1: What is CNTF mainly used for?

CNTF is primarily used for motor neuron survival, astrocyte culture, retinal protection, and neurodegenerative disease research.

Q2: Is this CNTF suitable for serum-free culture systems?

Yes. EastMabBio recombinant CNTF is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant CNTF be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.