Recombinant Human Transferrin (APO) Instruction

General description

Apo-Transferrin is the iron-free form of transferrin, an iron-binding glycoprotein that serves as a carrier for ferric iron delivery to cells. It is used to control iron availability in cell culture and to prepare iron-loaded transferrin for specific applications. Recombinant Human Apo-Transferrin Protein (Y04504) is expressed from mammalian cells. It contains AA Val 20 - Pro 698 (Accession # P02787), and is widely used in advanced cell culture, iron metabolism research, and media formulation.

Application

· Iron-Controlled Cell Culture — Enables precise regulation of iron availability in defined media.

· Holo-Transferrin Preparation — Serves as a precursor for generating iron-loaded transferrin.

· Iron Metabolism and Uptake Studies — Investigates transferrin receptor-mediated endocytosis and iron trafficking.

· Serum-Free Media Formulation — Provides a defined iron carrier for chemically defined culture systems.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

Apo-transferrin is the iron-free form of transferrin that binds two ferric iron atoms with high affinity to become holo-transferrin. This iron-binding capacity makes transferrin essential for regulating free iron levels and delivering iron to proliferating cells. In cell culture, apo-transferrin allows researchers to control iron supplementation precisely, preventing the toxic effects of free iron while ensuring adequate iron availability for DNA synthesis and metabolism.

Q&A

Q1: What is Apo-Transferrin mainly used for?

Apo-Transferrin is primarily used for iron-controlled cell culture, holo-transferrin preparation, iron metabolism studies, and serum-free media formulation.

Q2: Is this Apo-Transferrin suitable for serum-free culture systems?

Yes. EastMabBio recombinant Apo-Transferrin is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant Apo-Transferrin be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.