Recombinant Bovine Transferrin (APO) Instruction

General description

Unlike Holo‑Transferrin (the iron‑saturated form), Apo‑Transferrin primarily acts as an iron scavenger – it competitively binds free iron, thereby precisely regulating the iron concentration in the culture medium.Both forms can bind to transferrin receptor 1 (TfR‑1), though Holo‑Transferrin exhibits higher affinity. Recombinant Bovine APO-Transferrin Protein (Y04544) is expressed from mammalian cells. It contains AA Val 20 - Pro 704 (Accession # Q29443), and is widely used in cell culture media supplementation, iron metabolism studies, and biopharmaceutical applications.

Application

Serum‑free / chemically defined media – Serves as a critical component in serum‑free and low‑serum media, providing iron‑transport function.

Cultured beef production – Replaces serum‑derived transferrin in chemically defined culture systems for bovine muscle satellite cells.

Bovine embryonic stem cell (bESC) culture – Maintains iron homeostasis and supports pluripotency in bESC culture.

Bovine mammary epithelial cell culture – Supports the growth and differentiation of milk‑producing cells.

Iron metabolism research – Studies cellular iron uptake, release, and regulation under iron‑deficient conditions.

Bovine fetal development research – Models iron transport and nutrient delivery during bovine pregnancy.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

Apo-transferrin is the iron-free form of transferrin that binds two ferric iron atoms with high affinity to become holo-transferrin. This iron-binding capacity makes transferrin essential for regulating free iron levels and delivering iron to proliferating cells. In cell culture, apo-transferrin allows researchers to control iron supplementation precisely, preventing the toxic effects of free iron while ensuring adequate iron availability for DNA synthesis and metabolism.

Q&A

Q1: What is the difference between Apo‑Transferrin and Holo‑Transferrin?

Apo‑Transferrin is the iron‑free form, which acts primarily as an iron scavenger – it competitively binds free iron to precisely control iron concentration in the medium. Holo‑Transferrin is the iron‑saturated form, continuously releasing iron. 

Q2: Is this PDGF-AB suitable for serum-free culture systems?

Yes. EastMabBio recombinant PDGF-AB is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant PDGF-AB be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.