Recombinant Human BMP-2 Instruction

General description

Bone Morphogenetic Protein 2 (BMP-2) is a potent osteoinductive growth factor that drives mesenchymal stem cell commitment to the osteoblast lineage. It is clinically approved for spinal fusion and bone fracture healing. Recombinant Human BMP-2 Protein (Y00421H) is expressed from E. coli cells. It contains AA Gln 283 - Arg 396 (Accession # P12643), and is widely used in advanced cell culture, bone tissue engineering, and regenerative medicine.

Application

· Osteogenic Differentiation — Induces robust bone matrix mineralization in MSC and osteoprogenitor cultures.

· Bone Tissue Engineering — Supports scaffold-based bone regeneration and craniofacial reconstruction.

· Spinal Fusion Research — Models intervertebral disc and spinal bone healing mechanisms.

· Cartilage and Tendon Repair Studies — Investigates BMP-2-mediated soft tissue regeneration.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

·36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in 4 mM HCl.

Backgroud

Bone Morphogenetic Protein 2 (BMP-2), also known as BMP2A or BDA2, is a member of the TGF-β superfamily and was the first growth factor demonstrated to induce de novo bone formation. BMP-2 signals through heterotetrameric complexes of BMP type I (ALK3/BMPRIA or ALK6/BMPRIB) and type II receptors, activating SMAD1/5/8-dependent transcription of osteogenic master regulators including RUNX2 and Osterix. Recombinant BMP-2 is the active pharmaceutical ingredient in several FDA-approved products for orthopedic applications. In research settings, BMP-2 serves as the gold standard for directing osteogenic differentiation of pluripotent stem cells and mesenchymal stem cells toward mature osteoblasts capable of producing mineralized extracellular matrix.

Q&A

Q1: What is BMP-2 mainly used for?

BMP-2 is primarily used for osteogenic differentiation, bone tissue engineering, spinal fusion research, and orthopedic regenerative medicine.

Q2: Is this BMP-2 suitable for serum-free culture systems?

Yes. EastMabBio recombinant BMP-2 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant BMP-2 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.