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Recombinant Human TGF-β1 Instruction

Recombinant Human TGF-β1

Cat#:Y04201N

Name:Recombinant Human TGF-β1

Species:Human

Purity:≥95%

Endotoxin: ≤10 EU/mg

Expression System:CHO

Size: 50μg 1mg

Price: $359.00 $2,540.00

Product Details
Generaldescription

Transforming Growth Factor Beta 1 (TGF-β1) is a multifunctional cytokine that regulates cell proliferation, differentiation, apoptosis, and immune responses. It plays a central role in tissue fibrosis, wound healing, and immune tolerance. Recombinant Human TGF-β1 Protein (Y04201N) is expressed from mammalian cells. It contains AA Ala 279 - Ser 390 (Accession # P01137), and is widely used in advanced cell culture, immunology research, and regenerative medicine applications.

Application

·  Regulatory T Cell Induction — Drives the differentiation of naïve CD4+ T cells into induced regulatory T cells (iTregs). 

·  Mesenchymal Stem Cell Differentiation — Promotes chondrogenic and osteogenic lineage commitment.

·  Epithelial-Mesenchymal Transition (EMT) Studies — Induces EMT in cancer and epithelial cell models.

·  Fibrosis and Tissue Repair Research — Models pathological fibrosis and wound healing mechanisms. 

·  Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Cat. No.

Y04201N

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).

Endotoxin

≤10 EU/mg by the LAL method.

Bioactivity

Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.1ng/mL. 

Accession

P01137

Alternative Names

Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB

Species

Human

Expression System

CHO

Mol. Weight

12.8 kDa

Expression Region

Ala279-Ser390

Tag Info

Tag free

Form

Lyophilized

Buffer

0.085% TFA, 30% ACN, 5% Mannitol, pH 2.5.

Lead Time

Products are in stock, but custom products may require further consultation.

Shipping

The product is shipped with blue ice.

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state; · 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in 4mM HCL.

Backgroud

Transforming Growth Factor Beta 1 (TGF-β1), also known as TGFB1 or DPD1, is the founding member of the TGF-β superfamily. TGF-β1 is secreted as a latent complex and becomes biologically active upon proteolytic cleavage and dissociation from its latency-associated peptide (LAP). It signals through heteromeric complexes of TGF-β type I (ALK5) and type II receptors, activating downstream SMAD2/3-dependent transcriptional programs. TGF-β1 is a potent immunosuppressive cytokine that maintains immune homeostasis and self-tolerance while also driving pathological tissue fibrosis in chronic diseases.

Q&A

Q1: What is TGF-β1 mainly used for? TGF-β1 is primarily used for Treg differentiation, MSC lineage specification, EMT induction, fibrosis modeling, and immune regulation studies.

Q2: Is this TGF-β1 suitable for serum-free culture systems? Yes. EastMabBio recombinant TGF-β1 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant TGF-β1 be stored? Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used? Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation. 

Q5: Do you provide QC documents? Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.

Data

SDS-PAGE

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Recombinant Human TGF-β1 (Cat. No. Y04201N) SDS-PAGE under reducing (R) condition.

Bioactivity

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Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.1ng/mL.

Lot-to-Lot Consistency

image.png

Highly consistent bioactivity between different production batches of Recombinant Human TGF-β1 (Cat. No. Y04201N).

SEC-HPLC

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