Recombinant Bovine TGF-β1 Instruction

General description

Transforming Growth Factor Beta 1 (TGF-β1) is a multifunctional cytokine that regulates cell proliferation, differentiation, apoptosis, and immune responses. It plays a central role in tissue fibrosis, wound healing, and immune tolerance. Recombinant Bovine TGF-β1 Protein (Y04201B) is expressed from mammalian cells. It contains AA Ala 279 - Ser 390 (Accession # P18341), and is widely used in cell agriculture, developmental biology, and veterinary biotechnology.

Application

· Cell-Cultured Beef Production — Supports the maturation and organization of muscle fibers and extracellular matrix in structured meat.

· Regulatory T Cell Induction — Drives the differentiation of naïve CD4+ T cells into induced regulatory T cells.

· Bovine Mesenchymal Stem Cell Differentiation — Promotes chondrogenic and osteogenic lineage commitment.

· Bovine Wound Healing and Tissue Repair — Models fibrosis and granulation tissue formation.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in 4mM HCL.

Backgroud

Transforming Growth Factor Beta 1 (TGF-β1), also known as TGFB1 or DPD1, is the founding member of the TGF-β superfamily. TGF-β1 is secreted as a latent complex and becomes biologically active upon proteolytic cleavage and dissociation from its latency-associated peptide (LAP). It signals through heteromeric complexes of TGF-β type I (ALK5) and type II receptors, activating downstream SMAD2/3-dependent transcriptional programs. TGF-β1 is a potent immunosuppressive cytokine that maintains immune homeostasis and self-tolerance while also driving pathological tissue fibrosis in chronic diseases.

Q&A

Q1: What is TGF-β1 mainly used for?

TGF-β1 is primarily used for Treg differentiation, MSC lineage specification, EMT induction, fibrosis modeling, and immune regulation studies.

Q2: Is this TGF-β1 suitable for serum-free culture systems?

Yes. EastMabBio recombinant TGF-β1 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant TGF-β1 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.