Recombinant Human IL-21 Instruction

General description

Interleukin-21 is a pleiotropic cytokine produced by follicular helper T cells that regulates B cell affinity maturation, plasma cell differentiation, and the generation of stem cell-like memory T cells. It holds significant promise for enhancing the efficacy and durability of adoptive cell therapies. Recombinant Human IL-21 Protein (Y02901) is expressed from mammalian cells. It contains AA His 25 - Ser 162 (Accession # Q9HBE4), and is widely used in advanced cell culture, immunology research, and cell therapy manufacturing.

Application

· Stem Cell Memory T Cell Generation — Promotes TSCM phenotype with enhanced in vivo persistence and anti-tumor efficacy.

· NK Cell Cytotoxicity Enhancement — Boosts antibody-dependent cellular cytotoxicity and target cell killing.

· B Cell and Plasma Cell Differentiation — Supports germinal center reactions and antibody production.

· CAR-T and TCR-T Cell Therapy Optimization — Improves therapeutic T cell product quality and durability.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

Interleukin-21, also known as IL-21, is a member of the IL-2 cytokine family that signals through a receptor complex composed of the IL-21Rα chain and the common γ-chain shared with IL-2, IL-4, IL-7, IL-9, and IL-15. This receptor architecture places IL-21 at the intersection of multiple immune signaling networks while conferring unique biological properties. IL-21 activates JAK1/JAK3-dependent STAT3 signaling with weaker STAT5 activation compared to IL-2, creating a distinctive transcriptional program that favors effector differentiation over regulatory suppression. In T cell biology, IL-21 is unique in its ability to promote the generation of stem cell memory T cells that exhibit superior in vivo persistence, proliferative capacity, and anti-tumor efficacy compared to terminally differentiated effector cells. In NK cell biology, IL-21 enhances cytotoxicity and ADCC, making it valuable for combination strategies with therapeutic antibodies. In B cell biology, IL-21 drives plasma cell differentiation and immunoglobulin production while also promoting apoptosis of incompletely activated B cells to maintain selection stringency. EastMabBio's IL-21 is validated for TSCM generation in primary human T cell cultures and supports the development of next-generation cell therapy products with enhanced therapeutic windows.

Q&A

Q1:  What is IL-21 mainly used for?

IL-21 is primarily used for stem cell memory T cell generation, NK cell cytotoxicity enhancement, B cell differentiation, and CAR-T cell therapy optimization.

Q2: Is this IL-21 suitable for serum-free culture systems?

Yes. EastMabBio recombinant IL-21 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant IL-21 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.