Recombinant Human LR3 IGF-1 Instruction

General description

LR3 IGF-1 is an analog of Insulin-Like Growth Factor 1 with an N-terminal extension and reduced binding to IGF binding proteins, resulting in significantly enhanced bioavailability and potency in cell culture. It is widely used as a superior alternative to native IGF-1 for promoting cell proliferation and survival. Recombinant Human LR3 IGF-1 Protein (Y03101N) is expressed from mammalian cells. It contains MFPAMPLSSLFVN + Gly 49 - Ala 118(E51R)(Accession # P05019), and is widely used in advanced cell culture, stem cell research, and bioprocessing.

Application

· Stem Cell and Primary Cell Expansion — Promotes robust proliferation of MSCs, fibroblasts, and epithelial cells with reduced protein concentration requirements.

· CHO Cell and Hybridoma Culture — Enhances cell density and productivity in bioreactor-based protein and antibody production.

· Neuronal Survival and Differentiation — Supports the growth and maturation of neural stem cells and primary neurons.

· Cell-Cultured Meat Production — Drives proliferation of muscle satellite cells and fibroblasts in serum-free media.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water

Backgroud

LR3 IGF-1 is an engineered analog of native IGF-1 containing a 13-amino acid N-terminal extension and a single amino acid substitution (Arg for Glu at position 3). These modifications reduce binding affinity to IGF binding proteins (IGFBPs) by approximately 1,000-fold while preserving full receptor binding activity through the IGF-1 receptor (IGF1R). This enhanced bioavailability results in significantly greater potency in cell culture, typically requiring 10-100 fold lower concentrations than native IGF-1 to achieve equivalent biological effects. LR3 IGF-1 activates PI3K-AKT and RAS-MAPK pathways to promote cell survival, proliferation, and protein synthesis. It is particularly valuable in serum-free and chemically defined media where IGFBPs are absent or minimal, allowing full exploitation of its enhanced receptor engagement.

Q&A

Q1: What is LR3 IGF-1 mainly used for?

LR3 IGF-1 is primarily used for stem cell expansion, CHO cell culture, neuronal survival, and cell-cultured meat production.

Q2: Is this LR3 IGF-1 suitable for serum-free culture systems?

Yes. EastMabBio recombinant LR3 IGF-1 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant LR3 IGF-1 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.