Recombinant Human IGF-2 Instruction

General description

Insulin-Like Growth Factor 2 is a fetal growth factor that promotes cell proliferation, survival, and differentiation during embryonic development. It is a potent mitogen for stem cells and supports the expansion of diverse progenitor populations. Recombinant Human IGF-II Protein (Y12441H) is expressed from mammalian cells. It contains AA Ala 25 - Glu 91 (Accession # P01344.1), and is widely used in advanced cell culture, developmental biology, and stem cell research.

Application

· Pluripotent Stem Cell Expansion — Supports self-renewal and proliferation of iPSCs and ESCs in defined conditions.

· Hematopoietic Progenitor Cell Culture — Enhances expansion and colony formation of bone marrow and cord blood cells.

· Adipogenic and Myogenic Differentiation — Promotes lineage commitment in mesenchymal stem cell differentiation.

· Fetal Tissue Development Modeling — Investigates IGF-II-driven growth mechanisms in developmental biology.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

• 36 months at -20°C to -80°C in lyophilized state;

• 6 months at -20°C to -80°C under sterile conditions after reconstitution;

• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

• Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

• It is recommended to redissolve in 4mM HCl.

Backgroud

IGF-2, also known as somatomedin A, is a 67-amino acid polypeptide that is highly expressed during fetal development and plays a major role in embryonic and placental growth. It signals through the IGF1R, IGF2R, and insulin receptor isoform A, with IGF2R functioning primarily as a clearance receptor that regulates IGF-2 bioavailability. IGF-2 is imprinted in most mammalian tissues, with paternal allele expression and maternal allele silencing, and dysregulation of this imprinting is associated with growth disorders and cancer. In cell culture, IGF-2 supports the expansion of pluripotent stem cells, hematopoietic progenitors, and mesenchymal stem cells, often in combination with other growth factors.

Q&A

Q1: What is IGF-2 mainly used for?

IGF-2 is primarily used for pluripotent stem cell expansion, hematopoietic progenitor culture, adipogenic and myogenic differentiation, and developmental biology research.

Q2: Is this  IGF-2 suitable for serum-free culture systems?

Yes. EastMabBio recombinant IGF-2 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant IGF-2 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.