Recombinant Human IGF-1 Instruction

General description

Insulin-Like Growth Factor 1 is a mitogenic polypeptide that mediates the growth-promoting effects of growth hormone and regulates cell proliferation, differentiation, and survival across diverse tissue types. It is structurally related to insulin and plays critical roles in development, tissue repair, and metabolism. Recombinant Human IGF-1 Protein (Y05341H) is expressed from mammalian cells. It contains AA Gly 49 - Ala 118 (Accession # P05019), and is widely used in advanced cell culture, stem cell research, and regenerative medicine.

Application

· Stem Cell and Progenitor Cell Expansion — Supports proliferation and maintenance of MSCs, neural stem cells, and hematopoietic progenitors.

· Muscle Cell Growth and Differentiation — Promotes myoblast proliferation and skeletal muscle regeneration.

· Bone and Cartilage Tissue Engineering — Enhances osteogenic and chondrogenic differentiation in scaffold-based constructs.

· Cell-Cultured Meat Production — Drives muscle satellite cell proliferation and supports myogenic differentiation.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

• 36 months at -20°C to -80°C in lyophilized state;

• 6 months at -20°C to -80°C under sterile conditions after reconstitution;

• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

• Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

• It is recommended to redissolve in sterile deionized water.

Backgroud

IGF-1, also known as somatomedin C, is a 70-amino acid polypeptide produced primarily in the liver in response to growth hormone stimulation. It shares approximately 50% sequence homology with insulin and signals through the IGF1R tyrosine kinase receptor, activating PI3K-AKT and RAS-MAPK pathways to promote cell survival, proliferation, and protein synthesis. IGF-1 also binds with low affinity to the insulin receptor, explaining some overlap in metabolic effects. In cell culture, IGF-1 supports the expansion of stem cells, primary cells, and established cell lines with reduced serum requirements. It is particularly important for muscle cell culture, bone tissue engineering, and applications requiring sustained anabolic signaling.

Q&A

Q1: What is IGF-1 mainly used for?

IGF-1 is primarily used for stem cell expansion, muscle cell culture, bone tissue engineering, and cell-cultured meat production.

Q2: Is this  IGF-1 suitable for serum-free culture systems?

Yes. EastMabBio recombinant IGF-1 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant IGF-1 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.