Recombinant Bovine FGF-2/bFGF

General description

Basic Fibroblast Growth Factor is a heparin-binding growth factor essential for maintaining pluripotency in stem cells, promoting proliferation of fibroblasts and endothelial cells, and supporting neural progenitor expansion. It is one of the most widely used growth factors in cell culture applications. Recombinant Bovine bFGF Protein (Y00331) is expressed from E. coli cells. It contains AA Pro10-Ser155 (Accession # P03969), and is widely used in cell agriculture, developmental biology, and veterinary biotechnology.

Application

· Cell-Cultured Beef Production — Drives proliferation of bovine muscle satellite cells, fibroblasts, and adipogenic precursors in serum-free media.

· Bovine Embryonic Stem Cell Maintenance — Sustains self-renewal and pluripotency of bESCs for germline modification and developmental studies.

· Bovine Muscle Development and Myogenesis — Models embryonic muscle patterning and supports myogenic differentiation protocols.

· Bovine Intestinal Organoid Culture — Supports 3D culture of bovine gut epithelium for nutrition and microbiome research.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

Basic Fibroblast Growth Factor, also known as FGF-2 or HBGF-2, is a member of the fibroblast growth factor family. It lacks a classical signal peptide but is exported through unconventional secretion pathways and binds to heparan sulfate proteoglycans in the extracellular matrix. FGF-2 signals through FGFR1, FGFR2, and FGFR3 tyrosine kinase receptors, activating RAS-MAPK, PI3K-AKT, and PLCγ pathways to drive cell cycle progression and survival. In stem cell biology, FGF-2 is the cornerstone of feeder-free culture systems, working in concert with TGF-β or Activin signaling to sustain pluripotency marker expression. Its broad mitogenic activity extends to fibroblasts, endothelial cells, smooth muscle cells, and neural progenitors, making it one of the most versatile growth factors in cell culture applications.

Q&A

Q1:  What is FGF-2/bFGF mainly used for?

FGF-2/bFGF is primarily used for pluripotent stem cell maintenance, neural progenitor expansion, fibroblast culture, and angiogenesis research.

Q2: Is this FGF-2/bFGF suitable for serum-free culture systems?

Yes. EastMabBio recombinant FGF-2/bFGF is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant FGF-2/bFGF be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.