Recombinant Human FGF-4 (E.coli-expressed)

General description

Fibroblast Growth Factor 4 is a developmentally regulated growth factor essential for limb bud formation, inner cell mass proliferation, and trophoblast differentiation. It supports the expansion of pluripotent stem cells and drives lineage specification in early embryogenesis. Recombinant Human FGF-4 Protein (Y00921H) is expressed from E. coli cells. It contains AA Ala 31 - Arg 206 (Accession # P08620), and is widely used in advanced cell culture, developmental biology, and stem cell research.

Application

· Pluripotent Stem Cell Expansion — Maintains self-renewal of embryonic stem cells in combination with other growth factors.

· Trophoblast Differentiation — Promotes the development of placental cell lineages from stem cell models.

· Limb Bud and Skeletal Development Research — Models embryonic patterning and chondrogenic specification.

· Endoderm and Hepatocyte Differentiation — Supports early endodermal lineage commitment and liver progenitor expansion.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

Fibroblast Growth Factor 4, also known as FGF-4 or K-FGF, is a member of the FGF family originally identified as a transforming gene in stomach cancer. During embryonic development, FGF-4 is expressed in the inner cell mass of the blastocyst, the apical ectodermal ridge of the limb bud, and the developing gut endoderm, where it regulates cell proliferation, survival, and positional identity. FGF-4 signals through FGFR1 and FGFR2 with high affinity, activating canonical RAS-MAPK and PI3K-AKT pathways. In stem cell biology, FGF-4 was historically used for maintaining mouse embryonic stem cell pluripotency before the widespread adoption of LIF and BMP-4 protocols. In human pluripotent stem cell research, FGF-4 continues to be valuable for studying trophoblast differentiation, modeling early placental development, and investigating the signaling networks that govern lineage commitment during gastrulation. FGF-4 also plays a role in endoderm specification and hepatoblast proliferation, making it relevant for liver tissue engineering and directed differentiation protocols. EastMabBio's mammalian-expressed FGF-4 ensures proper protein folding and bioactivity for these demanding developmental biology applications.

Q&A

Q1:  What is FGF-4 mainly used for?

FGF-4 is primarily used for pluripotent stem cell expansion, trophoblast differentiation, limb bud development research, and endoderm specification.

Q2: Is this FGF-4 suitable for serum-free culture systems?

Yes. EastMabBio recombinant FGF-4 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant FGF-4 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.