Recombinant Human PDGF-BB Instruction

General description

Platelet-Derived Growth Factor BB (PDGF-BB) is a potent mitogen for mesenchymal cells, driving fibroblast, smooth muscle cell, and glial cell proliferation. It plays critical roles in wound healing, angiogenesis, and pericyte recruitment. Recombinant Human PDGF-BB Protein (Y03411) is expressed from mammalian cells. It contains AA Ser 82 - Thr 190 (Accession # P01127), and is widely used in advanced cell culture, vascular biology, and regenerative medicine research.

Application

· Mesenchymal Stem Cell Expansion — Supports robust proliferation and maintenance of multipotency.

· Smooth Muscle Cell Culture — Promotes growth and phenotypic modulation of vascular smooth muscle cells.

· Pericyte Recruitment and Angiogenesis Studies — Models blood vessel maturation and microvascular stability.

· Wound Healing and Fibrosis Research — Investigates tissue repair mechanisms and pathological scarring.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

Platelet-Derived Growth Factor BB (PDGF-BB), also known as SIS or SSV, is a homodimeric member of the PDGF family. PDGF-BB is the most broadly active PDGF isoform, capable of binding all PDGF receptor combinations (PDGFR-αα, PDGFR-αβ, and PDGFR-ββ) with high affinity. It is a critical survival factor for pericytes and regulates vascular permeability and blood-brain barrier integrity. Genetic deletion of PDGF-B in mice results in lethal vascular abnormalities due to pericyte deficiency. In cell culture, PDGF-BB serves as an essential growth supplement for mesenchymal stem cell expansion, neural crest-derived cell maintenance, and fibroblast proliferation assays.

Q&A

Q1: What is PDGF-BB mainly used for?

PDGF-BB is primarily used for MSC expansion, smooth muscle cell culture, angiogenesis research, and wound healing studies.

Q2: Is this PDGF-BB suitable for serum-free culture systems?

Yes. EastMabBio recombinant PDGF-BB is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant PDGF-BB be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.