Recombinant Human PDGF-AB Instruction

General description

Platelet-Derived Growth Factor AB is a heterodimeric isoform that signals through PDGFR-αα and PDGFR-αβ receptors, combining the cellular targets of PDGF-AA and PDGF-BB. It is a potent mitogen for mesenchymal cells and supports diverse tissue repair processes. Recombinant Human PDGF-AB Protein (Y12101) is expressed from mammalian cells. It contains AA Ser 87 - Thr 211 (α-chain) and Ser 82 - Thr 190 (β-chain) (Accession # P04085 & P01127), and is widely used in advanced cell culture, tissue engineering, and regenerative medicine.

Application

· Mesenchymal Cell Proliferation — Drives growth of fibroblasts, smooth muscle cells, and osteoblasts.

· Wound Healing and Tissue Regeneration — Supports granulation tissue formation and angiogenesis.

· Bone and Periodontal Tissue Repair — Promotes osteoblast proliferation and matrix mineralization.

· Vascular Remodeling Studies — Models smooth muscle cell recruitment and vessel wall thickening.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

PDGF-AB is a heterodimeric isoform containing one A-chain and one B-chain subunit, enabling it to bind PDGFR-αα and PDGFR-αβ receptor complexes but not PDGFR-ββ homodimers. This intermediate receptor specificity expands its cellular targets beyond PDGF-AA while maintaining distinct properties from the broadly active PDGF-BB. PDGF-AB is stored in platelet α-granules and released during clotting, contributing to wound healing and tissue repair. In cell culture, it supports the expansion of diverse mesenchymal cell types and is frequently used in combination with other growth factors for tissue engineering applications.

Q&A

Q1: What is PDGF-AB mainly used for?

PDGF-AB is primarily used for mesenchymal cell proliferation, wound healing research, bone repair studies, and vascular remodeling modeling.

Q2: Is this PDGF-AB suitable for serum-free culture systems?

Yes. EastMabBio recombinant PDGF-AB is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant PDGF-AB be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.