Recombinant Human FGF-8b Instruction

Generaldescription

Fibroblast Growth Factor 8b (FGF-8b) is a key member of the FGF family involved in embryonic development, neural patterning, cell proliferation, and tissue morphogenesis. Recombinant Human FGF-8b Protein (Y06021H) is expressed from E. coli cells. It contains AA Gln 23 - Arg 215 (Accession # P55075-3), and is widely used in advanced cell culture and developmental biology research.

Application

·Dopaminergic Neuron Differentiation— Promotes differentiation of stem cells into dopaminergic neurons.

·Brain Organoid Culture— Supports neural patterning and organoid development.

·Stem Cell Culture— Enhances cell differentiation and lineage specification.

·Developmental Biology Research— Regulates embryonic and neural development pathways.

·Serum-Free Culture Systems— Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

·36 months at -20°C to -80°C in lyophilized state;

·6 months at -20°C to -80°C under sterile conditions after reconstitution;

·7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

·Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

·It is recommended to redissolve in sterile deionized water.

Backgroud

Fibroblast Growth Factor 8 (FGF8), also known as AIGF or HBGF-8, is a member of the fibroblast growth factor (FGF) family. FGF family proteins exhibit broad mitogenic and cell survival activities and participate in multiple biological processes, including embryonic development, cell proliferation, morphogenesis, tissue repair, and neural patterning. Among its isoforms, FGF-8b is considered one of the most biologically active variants and plays a crucial role in midbrain development and neuronal differentiation.

Q&A

Q1:What is FGF-8b mainly used for?

FGF-8b is primarily used for neural differentiation, stem cell culture, organoid development, and developmental biology research.

Q2:Is this FGF-8b suitable for serum-free culture systems?

Yes. EastMabBio recombinant FGF-8b is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3:How should recombinant FGF-8b be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4:What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5:Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.