Recombinant Human FGF-10 Instruction

General description

Fibroblast Growth Factor 10, also known as Keratinocyte Growth Factor 2, is a mesenchymal-derived growth factor that regulates epithelial morphogenesis, branching, and wound healing. It is essential for lung, prostate, and mammary gland development. Recombinant Human FGF-10 Protein (Y01121H) is expressed from E. coli cells. It contains AA Gln 38 - Ser 208 (Accession # O15520), and is widely used in advanced cell culture, organoid research, and tissue engineering.

Application

· Lung Organoid Development — Promotes branching morphogenesis in airway and alveolar organoid cultures.

· Prostate and Mammary Epithelial Culture — Supports epithelial cell proliferation and morphogenesis.

· Wound Healing and Re-epithelialization — Enhances keratinocyte migration and tissue repair.

· Hepatocyte Expansion — Supports liver progenitor cell proliferation in serum-free conditions.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

It is recommended to redissolve in sterile deionized water.

Backgroud

Fibroblast Growth Factor 10, also known as FGF-10 or KGF2, is a member of the FGF7 subfamily that signals exclusively through FGFR2IIIb, an epithelial-specific receptor isoform. This restricted receptor distribution underlies the paracrine signaling mechanism by which mesenchymal-derived FGF-10 regulates epithelial behavior during development and tissue repair. FGF-10 is essential for limb bud formation, lung branching morphogenesis, and prostate development, with genetic deletion resulting in perinatal lethality due to lung agenesis. In organoid culture, FGF-10 serves as a critical component of lung organoid media, promoting branching morphogenesis in both proximal airway and distal alveolar models. Its ability to stimulate epithelial proliferation without mesenchymal cell expansion makes it particularly valuable for studying pure epithelial behaviors in defined culture conditions.

Q&A

Q1:  What is FGF-10 mainly used for?

FGF-10 is primarily used for lung organoid development, prostate and mammary epithelial culture, wound healing research, and hepatocyte expansion.

Q2: Is this FGF-10 suitable for serum-free culture systems?

Yes. EastMabBio recombinant FGF-10 is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant FGF-10 be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.