Recombinant Human RANK L Instruction

General description

Receptor Activator of Nuclear Factor Kappa-B Ligand is a TNF superfamily cytokine essential for osteoclast differentiation, lymph node development, and mammary gland morphogenesis. It is a therapeutic target in osteoporosis and bone metastasis. Recombinant Human RANKL Protein (Y07101) is expressed from mammalian cells. It contains AA Gly 64-Asp 245 (Accession # Q5T9Y4), and is widely used in advanced cell culture, bone biology, and cancer research.

Application

· Osteoclast Differentiation — Drives the formation of TRAP-positive multinucleated osteoclasts from monocyte precursors.

· Bone Resorption and Remodeling Research — Models physiological and pathological bone turnover.

· Tumor-Induced Bone Destruction — Investigates RANKL-mediated osteolysis in skeletal metastasis.

· Dendritic Cell and T Cell Activation — Modulates immune cell function through RANK signaling.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

RANKL is a type II transmembrane protein of the TNF superfamily that signals through the RANK receptor, activating NF-κB, MAPK, and PLCγ pathways. It is produced by osteoblasts, stromal cells, and activated T cells. RANKL is indispensable for osteoclastogenesis, with genetic deletion causing severe osteopetrosis and absence of tooth eruption. Clinically, denosumab (anti-RANKL) is used to treat osteoporosis, giant cell tumor of bone, and skeletal-related events in cancer. In research, RANKL is the standard stimulus for generating osteoclasts from monocytes or bone marrow cultures.

Q&A

Q1: What is RANKL mainly used for?

RANKL is primarily used for osteoclast differentiation, bone resorption research, tumor bone destruction modeling, and immune cell activation.

Q2: Is this RANKL suitable for serum-free culture systems?

Yes. EastMabBio recombinant RANKL is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant RANKL be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.