Recombinant Human TNFα Instruction

General description

Tumor Necrosis Factor Alpha is a pleiotropic pro-inflammatory cytokine that regulates immune responses, cell survival, and apoptosis. It is a master regulator of inflammation and a therapeutic target in autoimmune diseases. Recombinant Human TNF-α Protein (Y04401) is expressed from mammalian cells. It contains AA Val 77 - Leu 233 (Accession # P01375), and is widely used in advanced cell culture, immunology research, and inflammatory disease modeling.

Application

· Macrophage and Monocyte Activation — Induces classical activation and pro-inflammatory cytokine production.

· NF-κB and Inflammation Signaling Research — Models TNF-α-driven inflammatory gene expression pathways.

· Apoptosis and Necroptosis Studies — Investigates cell death mechanisms through TNFR1 and TNFR2 signaling.

· Intestinal Organoid Barrier Dysfunction — Induces inflammatory phenotypes mimicking Crohn's disease and colitis.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

TNF-α is produced primarily by activated macrophages and signals through TNFR1 and TNFR2, activating NF-κB, MAPK, and death domain pathways. It is a central mediator of acute and chronic inflammation, with therapeutic antibodies (infliximab, adalimumab, etanercept) revolutionizing the treatment of rheumatoid arthritis, inflammatory bowel disease, and psoriasis. In cell culture, TNF-α is used to activate macrophages, model inflammatory signaling, study cell death pathways, and induce barrier dysfunction in intestinal organoids.

Q&A

Q1: What is TNF-α mainly used for?

TNF-α is primarily used for macrophage activation, inflammation signaling research, apoptosis studies, and intestinal organoid modeling.

Q2: Is this TNF-α suitable for serum-free culture systems?

Yes. EastMabBio recombinant TNF-α is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant TNF-α be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.