Recombinant Human IFNγ Instruction

General description

Interferon Gamma is a type II interferon that orchestrates cell-mediated immunity, macrophage activation, and antiviral responses. It is essential for Th1 polarization, M1 macrophage polarization, and tumor immunosurveillance. Recombinant Human IFN-γ Protein (Y01801) is expressed from mammalian cells. It contains AA Gln 24 - Gln 166 (Accession # P01579), and is widely used in advanced cell culture, immunology research, and cell therapy quality control.

Application

· Th1 Cell Polarization — Drives T-bet expression and IFN-γ production in activated CD4+ T cells.

· M1 Macrophage Activation — Induces classical activation with enhanced antigen presentation and microbicidal activity.

· Tumor Immunosurveillance Research — Models IFN-γ-mediated tumor rejection and immune checkpoint dynamics.

· Cell Therapy Potency Assays — Validates T cell functionality and serves in release criteria for therapeutic products.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

IFN-γ is produced primarily by NK cells and activated T cells. It signals through the IFNGR1/IFNGR2 receptor complex, activating JAK1/JAK2-dependent STAT1 signaling that drives the transcription of interferon-stimulated genes. IFN-γ is the principal activator of macrophage microbicidal functions, enhances MHC class I and II expression, and promotes Th1 differentiation while suppressing Th2 responses. In cell therapy manufacturing, IFN-γ is employed in potency assays, release criteria, and the induction of PD-L1 expression for immune checkpoint studies.

Q&A

Q1: What is IFN-γ mainly used for?

IFN-γ is primarily used for Th1 polarization, M1 macrophage activation, tumor immunosurveillance research, and cell therapy potency assays.

Q2: Is this IFN-γ suitable for serum-free culture systems?

Yes. EastMabBio recombinant IFN-γ is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant IFN-γ be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.