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Recombinant Human EGF

Recombinant Human EGF

Cat#:H004D/H004L

Species:Human

Size:1L

Purity:≥95%

Endotoxin: ≤10 EU/mg

Application Details
Cat. No.

H004D/H004L

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Concentration

1000ppm

Bioactivity

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤0.3 ng/mL.

Accession

P01133

Alternative Names

Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone

Species

Human

Expression System

CHO

Target Protein Sequence

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

Mol. Weight

6.2 kDa

Expression Region

Asn971-Arg1023

Tag Info

Tag free

Formulation

Lyophilized

Buffer
PBS,5% mannitol and 0.01% Tween 80, pH7.4
Size

1L

Lead Time

Products are in stock,but custom products may require further consultation

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice

Storage & Stability

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Application

Anti-aging,Anti-wrinkle,Skin lifting,Wound healing,etc.

Data
SDS-PAGE
image.png

Recombinant Human EGF (Cat. No. H004D/H004L)  SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with H004D/H004L SDS-PAGE.

Bioactivity
EGF-1_00.jpg

Recombinant Human EGF (Cat. No. H004D/H004L) stimulates proliferation of NIH3T3 cells. The ED50 for this effect  ≤0.3 ng/mL. The acivity of H004D/H004L was equivalent to other competing products.

Bioactivity
EGF-2_00.jpg

Highly consistent bioactivity between different production batches of Recombinant Human EGF (Cat. No. H004D/H004L).

SEC-HPLC
image.png

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