EastMabBio K005 Purification Kit | Single Spin for Highly Active NK Cells – Effortless Isolation

Still troubled by the low purity of conventional density gradient separation?
Frustrated by the tedious processes of magnetic bead sorting or flow cytometry?
Struggling with tight schedules, high equipment demands, and poor cell viability?

Try Dongkang's newly launched K005 Human NK Cell Purification Kit—Save time, effort, and your budget!

Principle of Action

The Human NK Cell Purification Cocktail cross-links non-target cells in human whole blood with multiple red blood cells (RBCs) to form immunorosettes. These rosettes increase the density of non-target cells, enabling them to sediment—along with free RBCs—during centrifugation with a density gradient medium. Target cells remain unlabeled by antibodies and are highly enriched, allowing for easy collection at the interface between the plasma layer and the density gradient medium.

01 Procedure

02 Product Highlights

Simple Operation

Based on the principle of density gradient centrifugation, simply add the K005 Human NK Cell Purification Cocktail to whole blood, incubate, and perform density gradient centrifugation. There is no need for expensive or complex magnetic or flow cytometry sorting equipment, saving time, effort, and budget.

Broad Sample Compatibility

Suitable for various sample types, including human peripheral blood and umbilical cord blood.

High-Purity Enrichment

Flow Cytometry Assessment of NK Cell (CD3⁻CD56⁺) Purity Before and After Sorting：

Excellent Cell Viability

The gentle, antibody-free labeling principle (target cells remain unbound by antibodies) ensures optimal post-sorting cell condition, enabling direct use in a variety of downstream applications.

Ready-to-Use

The enriched NK cells require no additional processing and can be immediately utilized for:

Flow cytometry analysis；Cell culture and functional studies (e.g., cytotoxicity assays)；DNA/RNA extraction and molecular biology analysis

03 Q&A

Q: What is the purity of NK cells after sorting?

A: One kit can process up to 50 mL of blood sample. If the sample volume is smaller (e.g., 10 mL), the experiment can still be performed by reconstituting the kit with 2 mL of sterile water and adding it at a concentration of 40 μL per mL of blood.

Q: What is the purity of NK cells after sorting?

A: Purity may vary among donors, typically ranging from 40% to 75% after purification. If the initial purity is low, continuing standard culture protocols can increase purity to over 80% by Day 6 and over 90% by Day 10.

Q: How should the sorted cells be seeded?

A: Depending on separation efficiency and blood sample quality:

A 10 mL blood sample typically yields 1–4 × 10⁶ cells

A 50 mL sample yields 0.5–2 × 10⁷ cells

For fresh blood samples, seed NK cells in a T25 flask with 9 mL of activation medium and 10% autologous plasma (≈1 mL), at a approximate density of 0.5–2 × 10⁶ cells/mL.

04 Applications

The Dongkang K005 NK Cell Purification Kit overcomes the limitations of high-cost magnetic bead-based negative selection and the low purity of traditional density gradient centrifugation. Whether in tumor immunology, infection immunology, cell therapy development, or basic NK cell biology research, rapidly obtaining high-purity, high-viability NK cells is the essential first step toward success!