Recombinant Human Fibronectin Instruction

General description

Fibronectin is a high-molecular-weight extracellular matrix glycoprotein that mediates cell adhesion, migration, proliferation, and differentiation. It serves as a critical substrate for maintaining pluripotency in human induced pluripotent stem cells and embryonic stem cells. Recombinant Human Fibronectin Protein (Y13142H) is expressed from mammalian cells and widely used in advanced cell culture, stem cell research, and cell therapy manufacturing.

Application

· iPSC and ESC Culture Substrate — Supports feeder-free maintenance of pluripotent stem cells in combination with defined media.

· Cell Adhesion and Spreading Assays — Promotes integrin-mediated attachment for diverse cell types.

· Neural Stem Cell Expansion — Enhances survival and proliferation of CNS-derived progenitor populations.

· Cell Therapy Manufacturing — Used in clinical-grade coating protocols for adherent cell production.

· Serum-Free Culture Systems — Suitable for chemically defined and xeno-free cell culture media.

Product Specifications

Stability and Reconstitution

· 36 months at -20°C to -80°C in lyophilized state;

· 6 months at -20°C to -80°C under sterile conditions after reconstitution;

· 7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

· Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

· It is recommended to redissolve in sterile deionized water.

Backgroud

Fibronectin, also known as FN1 or CIG, is a dimeric glycoprotein present in plasma and extracellular matrices. It contains multiple functional domains that bind integrins, collagen, fibrin, and heparan sulfate proteoglycans, enabling complex matrix assembly and cell-matrix communication. The central cell-binding domain containing the RGD motif interacts with α5β1 integrin to trigger focal adhesion formation and downstream FAK-Src signaling. In stem cell biology, fibronectin coating replaces undefined feeder layers, enabling fully defined, xeno-free culture conditions essential for clinical translation of iPSC-derived therapies. Fibronectin also plays critical roles in wound healing, embryonic morphogenesis, and tumor metastasis.

Q&A

Q1: What is Fibronectin mainly used for?

Fibronectin is primarily used for iPSC/ESC feeder-free culture, cell adhesion promotion, neural stem cell expansion, and clinical cell therapy manufacturing.

Q2: Is this Fibronectin suitable for serum-free culture systems?

Yes. EastMabBio recombinant Fibronectin is compatible with serum-free, xeno-free, and chemically defined media systems.

Q3: How should recombinant Fibronectin be stored?

Lyophilized protein should be stored at -20°C or below. After reconstitution, aliquot and avoid repeated freeze-thaw cycles.

Q4: What shipping conditions are used?

Products are shipped on dry ice with validated cold-chain packaging to maintain stability during international transportation.

Q5: Do you provide QC documents?

Yes. COA, MSDS, TSE-BSE statements, endotoxin reports, bioactivity data, and additional QC documentation are available upon request.