Recombinant Human Fibronectin: The Essential Biomaterial for Cell Culture and Regenerative Medicine

1. What is Recombinant Human Fibronectin (rhFN)?

Fibronectin (FN) is a high-molecular-weight glycoprotein widely present in the extracellular matrix (ECM), characterized by a V-shaped dimeric structure connected by two disulfide bonds. As one of the primary cell adhesion molecules, fibronectin plays an irreplaceable role in critical physiological processes including embryogenesis, wound healing, hemostasis, and thrombosis.

Recombinant Human Fibronectin (rhFN) is human-derived fibronectin produced through genetic recombination technology in in vitro expression systems. Compared to natural fibronectin extracted from animal plasma, rhFN offers higher purity, superior batch-to-batch consistency, lower immunogenicity risk, and is completely free from animal-source pathogen contamination, making it the preferred choice for both research and industrial applications.

2. EastMabBio: A Manufacturer of Recombinant Protein Raw Materials in China

EastMabBio was established in 2016 and is headquartered in Nantong, Jiangsu Province. The company is a national high-tech enterprise dedicated to providing high-quality recombinant protein raw materials globally. Founded by a team of scientists with extensive backgrounds in antibody drug research and development, EastMabBio adheres to the mission of "Improving Life and Health" and has become an industry benchmark through years of dedicated effort in the recombinant protein field.

2.1 Corporate Strength

- R&D Platform: Invested nearly 100 million RMB to build recombinant protein R&D, validation, and production technology platforms

- Production Scale: Over 10,000 square meters of facilities in Nantong and Suzhou, equipped with up to 1,000-liter fermenters and nearly 4,000 square meters of GMP workshops

- Production Capacity: Capable of kilogram-scale recombinant protein production

- Quality Certifications:SGS-GMP, ISO13485, ISO9001, ISO14001, and ISO45001 certified; recognized as Jiangsu Province "Specialized, Refined, Unique, and Innovative" (SRUI) enterprise

- Technology Licensing: One of the few companies in the industry with global commercial licensing for CHO cell technology

2.2 Product Portfolio

EastMabBio product lines cover three core domains:

-Cell Culture Protein Raw Materials: GMP-grade cytokines, growth factors, transferrin, fibronectin, CD series antibodies, and more

-IVD Diagnostic Proteins: Respiratory series, infectious disease series, gastrointestinal series antigens and antibodies

-Medical/Diagnostic Enzymes: Isothermal amplification enzymes, gene editing enzymes, universal nucleases, and more

3. EastMabBio Recombinant Human Fibronectin Product Details

3.2 Key Product Advantages

HEK293 Expression System: Human cell line expression ensures protein folding and post-translational modifications closer to native human fibronectin

- High Purity: ≥95% purity verified by SDS-PAGE and SEC-HPLC

- Low Endotoxin: ≤10 EU/mg, meeting stringent requirements for stem cell and immune cell culture

- High Bioactivity: ED₅₀ ≤ 400 ng/mL, ensuring excellent cell adhesion promotion

- Sterile Packaging: 0.2μm filtration for sterility, safeguarding culture system integrity

4. Core Applications of Recombinant Human Fibronectin in Cell Culture

4.1 Promoting Cell Adhesion and Spreading

Fibronectin is one of the most commonly used matrix coating proteins in cell culture. Research has shown that on fibronectin-coated culture surfaces, the adhesion rate of various cell types (such as BHK, CHO, mesenchymal stem cells, etc.) can increase from 10% in control groups to over 90%. Fibronectin binds to integrin receptors on the cell surface through its classic RGD sequence, activating downstream signaling pathways and promoting cytoskeletal reorganization and cell spreading.

4.2 Enhancing Cell Proliferation and Metabolism

Fibronectin not only promotes cell adhesion but also activates PI3K/Akt and MAPK signaling pathways through continuous interaction with cell surface receptors, promoting cell cycle progression. Experimental data shows that in fibronectin-coated culture systems, cell DNA, RNA, and protein synthesis rates can increase by approximately 5-fold, with significantly higher colony formation rates.

4.3 Supporting Stem Cell Culture

In stem cell culture, fibronectin can serve as an alternative to Matrigel or feeder cells, providing a chemically defined culture environment for embryonic stem cells (ES) and induced pluripotent stem cells (iPS). Fibronectin coating maintains the undifferentiated state and self-renewal capacity of stem cells while facilitating subsequent enzymatic dissociation for passaging.

4.4 Immune Cell Culture and CAR-T Manufacturing

In immune cell therapy applications such as CAR-T cell manufacturing, fibronectin fragments (such as RetroNectin/CH-296) act as a "molecular bridge" between viral vectors and T cells by binding to VLA-4 and VLA-5 integrins, increasing retroviral or lentiviral transduction efficiency to 30–70%, significantly improving CAR-T cell production efficiency.

5. Why Choose EastMabBio Recombinant Human Fibronectin?

6. Frequently Asked Questions (FAQ)

Q1: Can the fibronectin coating solution be reused? 

The coating working solution can be reused within one week when stored at 4°C, but fresh preparation is recommended for optimal results.

Q2: Which cell types is fibronectin suitable for? 

Fibronectin is suitable for most adherent cells, including mesenchymal stem cells, endothelial cells, fibroblasts, epithelial cells, neural cells, and various tumor cell lines.

Q3: Can EastMabBio products be used for in vivo experiments? 

 EastMabBio products are positioned as recombinant proteins for cell culture and are not intended for direct injection or any in vivo experiments. Please select appropriate grade products for in vivo applications.

Q4: How can I contact EastMabBio to obtain products? 

 You can contact EastMabBio through the official website (www.eastmab.com) or email (product@eastmab.com) to obtain product catalogs and technical support.

7. Conclusion

EastMabBio Recombinant Human Fibronectin (Cat# Y13142H), with its HEK293 human expression system, ≥95% high purity, and ≤10 EU/mg low endotoxin core advantages, has become the preferred matrix protein for cell culture, stem cell research, immune cell therapy, and other fields. Whether for cell adhesion optimization in basic research or CAR-T cell manufacturing in industrialization, EastMabBio rhFN provides reliable assurance for your experiments.

Contact EastMabBio for free samples and technical support!