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Y00801 Recombinant Human EGF

Y00801 Recombinant Human EGF

Cat#:Y00801

Species:Human

Size:10μg/50μg/1mg

Purity:≥95%

Endotoxin: ≤10 EU/mg

Application Details
Cat. No.

Y00801

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Bioactivity

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤0.3 ng/mL.

Accession

P01133

Alternative Names

Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone

Species

Human

Expression System

CHO

Target Protein Sequence

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

Mol. Weight

6.2 kDa

Expression Region

Asn971-Arg1023

Tag Info

Tag free

Formulation

Lyophilized

Buffer

PBS,5% mannitol and 0.01% Tween 80, pH7.4

Size

10μg/vail 50μg/vail 1mg/vail

Lead Time

Products are in stock, but custom products may require further consultation.

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at -20°C to -80°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Data

SDS-PAGE

image.png

Human EGF (Cat. No. Y00801)  SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y00801 SDS-PAGE

Bioactivity

Y00801-1.jpg

Human EGF (Cat. No. Y00801) stimulates proliferation of NIH3T3 cells. The ED50 for this effect ≤0.3 ng/mL. The bioacivity of Y00801 was equivalent to other competing products.

Bioactivity
Y00801-2.jpg
Highly consistent bioactivity between different production batches of Recombinant Human Transferrin (Cat. No. Y04501).

SEC-HPLC

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东抗生物