Antibody Discovery

Single cell antibody technology    Technology:  single cell antibody technology (single B cell antibody technology) to isolate individual human B lymphocytes and obtain fully human monoclonal antibodies from their genomes is the latest technology for antibody discovery. This method retains the natural pairing of the variable regions of the light and heavy chains, and has the advantages of good gene diversity, high efficiency, fully human source, and less cell required.

Single cell antibody technology

Technology:

single cell antibody technology (single B cell antibody technology) to isolate individual human B lymphocytes and obtain fully human monoclonal antibodies from their genomes is the latest technology for antibody discovery. This method retains the natural pairing of the variable regions of the light and heavy chains, and has the advantages of good gene diversity, high efficiency, fully human source, and less cell required.

Technical advantages:

1. All-human antibody;

2. The gene pool is large and the screening efficiency is high;

Nano-antibody technology

Technology:

nano-antibody (nanobody, Nb) is derived from heavy-chain antibody (HCAb) with natural missing light chains in camels, alpacas, sharks and other animals. Its molecular diameter reaches 2.2 nm at the nanometer level, so it is called nano-antibody. Nanobodies have high water solubility, high heat resistance and stability.

Technical advantages:

1. Alpaca breeding base has been established with stable and reliable sources;

2. Rapid screening platform for nano-antibodies, with guaranteed time;

3. Large-capacity humanized nano-antibody  library with high screening efficiency.

Antibody library technology-(phage, yeast, mammalian cell) display technology

Technology:

antibody library technology is to introduce foreign genes into phages, yeast cells or mammalian cells. Foreign proteins or peptides are fused with phage coat proteins or Aga2 proteins on yeast cell walls to display on phage/cell surfaces and maintain specific spatial conformation. This technique is suitable for large-scale studies of intermolecular interactions, especially in the fields of antibody discovery and epitope research. At present, some proteins and antibodies discovered or modified by phage display technology have been approved for clinical application. While yeast/mammalian cells in which chaperone proteins, folding enzymes and quality control mechanisms ensure that only properly folded proteins are secreted.

Technical advantages:

1. The antibody screening speed is fast, and 100,000 cells can be sorted in 3 minutes to obtain 100 candidate clones. (yeast display system);

2. Large-capacity humanized antibody library with high screening efficiency.

Single cell sequencing technology

Technology:

Single-cell sequencing (Single-cell sequencing) is different from ordinary sequencing in that the RNA (or DNA) extracted by ordinary sequencing originates from multiple cells in the sample, so the results of ordinary sequencing will inevitably be affected by heterogeneity between different cells. (Heterogeneity), while single-cell sequencing is to sequence the genome of a single cell, which can better help us understand the differences between cells. In antibody discovery, sequencing a single B cell can help us obtain a fully human antibody library with good genetic diversity.